赫斯特荧光染料 33258

中文名称:

赫斯特荧光染料 33258

中文同义词:

赫斯特荧光燃料，33258;甲嗪双苯咪酚;双苯咪唑;HOECHST 33258细胞蓝色荧光染料;HOECHST 33258细胞核染料;HOECHST 33258 荧光染料;33258荧光染料;HOECHST 33258细胞核染料*20MM水溶液*

英文名称:

Hoechst 33258

英文同义词:

Bis-BenzimideFluorochrome;p-(5-(5-(4-Methyl-1-piperazinyl)-1H-2-benzimidazolyl)-1H-2-benzimidazolyl)phenol trihydrochloride;Hoechst(R) 33258,98%;bisBenzimide, 2μ-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi-1H-benzimidazole hydrate trihydrochloride;HOE 33258, Hoechst 33258, 2μ-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride;4-[5-[6-(4-Methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride;Phenol, p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2-benzimidazolyl)-, trihydrochloride;Pibenzimol HCl

CAS号:

23491-45-4

分子式:

C25H27Cl3N6O

分子量:

533.88

EINECS号:

245-690-6

相关类别:

生化试剂-色素类;化学试剂;细胞生物学试剂;色素类;荧光探针，标记，颗粒和染色;试剂;细胞分析;生化试剂;细胞生物学;其他生化试剂;细胞信号和神经生物学;细胞死亡;细胞相关;分析试剂-显色剂;生物化工

Mol文件:

23491-45-4.mol

熔点

314 °C

密度

1.5099 (rough estimate)

折射率

1.6300 (estimate)

储存条件

-20°C

溶解度

H2O: 10 mg/mL

形态

powder

水溶解性

SOLUBLE

BRN

4088183

CAS 数据库

23491-45-4(CAS DataBase Reference)

EPA化学物质信息

Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5-bi-1H-benzimidazol]-2-yl]-, trihydrochloride (23491-45-4)

生物活性

Hoechst 33258 trihydrochloride (bisBenzimide H 33258 trihydrochloride) 是一种荧光染料，用于 DNA 染色。

靶点

IC50: 51.31±4.56 μM (HeLa cell), 32.43±3.27 μM (HL60 cell), 15.42±2.16 μM (U937 cell)

体外研究

Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC 50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI 50 from 84×10 -6 to 191.5×10 -6 mol/dm 3 . Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1.

用途

细胞遗传学研究的常用试剂，荧光染色细胞的DNA ，插入DNA的A-T区,具有低的细胞毒性,形成膜渗透性荧光DNA链。

危险品标志

危险类别码

22-36/38-36/37/38-20/21/22

安全说明

26-36-24/25-23

WGK Germany

RTECS号

SM1140500

3-8-10

海关编码