碘化丙啶

中文名称:

碘化丙啶

中文同义词:

碘化丙啶;碘化丙碇;碘化丙啶 PROPIDIUM IODIDE;PI染色液;碘化丙啶PI *10MM水溶液*;(碘化丙碇)PI 10MG;碘化丙啶(碘化丙锭、PI);碘丙啶

英文名称:

Propidium iodide

英文同义词:

3,8-DIAMINO-5-(3-DIETHYLAMINOPROPYL)-6-PHENYLPHENANTHRIDINIUM IODIDE METHIODIDE;3,8-DIAMINO-5-(3-DIETHYL-AMINOPROPYL)-6-PHENYLPHENANTHRIDIUM IODIDE METHIODIDE;3,8-DIAMINO-5-(3-[DIETHYLMETHYLAMMONIO])-6-PHENYLPHENAN-THRIDINIUM DIIODIDE;3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM;3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM DIIODIDE;3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenyl-phenanthridiniudii;3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridiniumdiiod;3,8-diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridiniumiodidemethiodi

CAS号:

25535-16-4

分子式:

C27H34IN4+

分子量:

541.5

EINECS号:

247-081-0

相关类别:

细胞生物学;色素类;荧光染料;细胞生物学试剂;化学试剂;小分子抑制剂;生物活性小分子;Aromatics Compounds;Aromatics;Intermediates Fine Chemicals;Pharmaceuticals;marker;Inhibitors;小分子抑制剂，天然产物;细胞信号和神经生物学;Phenanthridine;生化试剂;生物化工;有机化学

Mol文件:

25535-16-4.mol

熔点

220-225 °C (dec.) (lit.)

密度

1.5072 (estimate)

储存条件

2-8°C

溶解度

Difficult to mix.

形态

Flaky Powder

颜色

Dark red

水溶解性

Soluble in water at 1mg/ml

最大波长(λmax)

493 nm, 535 nm

敏感性

Light Sensitive Hygroscopic

BRN

3843838

生物领域应用

Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer

主要应用

Electroluminescent displays; photoresists

CAS 数据库

25535-16-4(CAS DataBase Reference)

简介

碘化丙啶是一种可对DNA染色的细胞核染色试剂，常用于细胞凋亡检测，英文全称是Propidium Iodide。它是一种溴化乙啶的类似物，在嵌入双链DNA后释放红色荧光。

生物活性

Propidium Iodide 是可用于细胞染色的红色荧光染料。

体外研究

Propidium Iodide is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids with a stoichiometry of one dye per 4-5 base pairs with little sequence preference. Propidium Iodide has evidenced of having no toxic effects on neurons, being today’s most common marker for membrane integrity and cell viability when applied prior to fixation (pre-fixation Propidium Iodide staining method). The pre-fixation staining has been widely used for quantitative assessments of neuronal cell decline in models of acute neurodegeneration, visualized as intensely labeled PI + -pycnotic nuclei of degenerating neurons . Propidium Iodide cannot cross the membrane of live cells, making it useful to measure the percentage of apoptotic cells by flow-cytometric analysis. The flow cytometric data shows an excellent correlation with the results obtained with both electrophoretic and colorimetric methods. This new rapid, simple and reproducible method proves useful for assessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus and lymph nodes.

用途

碘化丙啶(PI)是一种溴化乙啶的类似物，它在嵌入双链DNA后释放红色荧光。尽管PI不能通过活细胞膜，但却能穿过破损的细胞膜而对核染色。PI经常被用来与Calcein-AM或者FDA等荧光化合物一起使用，能同时对活细胞和死细胞染色。

危险品标志

Xi,Xn

危险类别码

36/37/38-68-67-48/20/22-40-36/38-22-20-63

安全说明

26-36-22-36/37

WGK Germany

RTECS号

SF7949600

3-8-10-23