远志皂苷D

中文名称:

远志皂苷D

中文同义词:

远志皂苷D;远志皂苷D2

英文名称:

Polygalacin D

英文同义词:

Polygalacin D;Onjisaponin D;Olean-12-en-28-oic acid, 3-(β-D-glucopyranosyloxy)-2,16,23-trihydroxy-, O-D-apio-β-D-furanosyl-(1→3)-O-β-D-xylopyranosyl-(1→4)-O-6-deoxy-α-L-mannopyranosyl-(1→2)-α-L-arabinopyranosyl ester, (2β,3β,4β,16α)-;Polygalacin D2

CAS号:

66663-91-0

分子式:

C57H92O27

分子量:

1209.34

EINECS号:

相关类别:

标准品;对照品;中药对照品;植物提取物;标准品，对照品;标准品-对照品

Mol文件:

66663-91-0.mol

密度 

1.54±0.1 g/cm3 (20 ºC 760 Torr)

酸度系数(pKa)

12.76±0.70(Predicted)

基本理化性质

化学式：C57H92O27 CAS号：66663-91-0 熔点： 221-226 比旋光度： -41.5 (c, 1 in MeOH) 分子量：1209.32000 外观： 白色针状晶体 纯度： ≥ 98% 溶解性： 可溶于水、乙醇、甲醇等溶剂，不溶于石油醚、氯仿、乙酸乙酯。 分子结构式：  

提取来源

远志科植物

用途

促进智力、减少心肌损伤、镇咳和祛痰作用。用于含量测定/鉴定/药理实验等。

储存条件

4℃冷藏、密封、避光

参考资料

http://www.chemsrc.com/cas/66663-91-0_69204.html http://www.shyuanye.com/goods-B21629.html

生物活性

Polygalacin D (PGD) 是从桔梗 Platycodon grandiflorum 中分离的具有抗癌和抗增殖特性的生物活性化合物。 Polygalacin D 抑制 IAP 蛋白家族的表达，包括存活蛋白，cIAP-1 和 cIAP-2 蛋白，并通过抑制 GSK3β，Akt 的磷酸化和PI3K 的表达来阻断 PI3K/Akt 途径。Polygalacin D 通过 PI3K/Akt 途径诱导凋亡 (apoptosis)。

靶点

IC50: IAP

体外研究

Polygalacin D (0-40 µM; 48 hours) inhibits cell proliferation with IC 50 s of 26.49 µM in the A549 cells and 20.52 µM in the H460 cells. Polygalacin D (0-20 µM; 48 hours) increases the proportion of early and late apoptotic cells, and nuclear condensation is observed in A549 and H460 cells. Polygalacin D (0-20 µM; 48 hours) may exerts its apoptotic effects by regulating the apoptotic proteins and the IAP family of proteins in A549 and H460 cells. Cell Proliferation Assay Cell Line: A549 and H460 cancer cell lines Concentration: 10 µM, 20 µM, 40µM Incubation Time: 48 hours Result: Inhibited the proliferation of NSCLC cell lines. Apoptosis Analysis Cell Line: A549 and H460 cancer cell lines Concentration: 10 µM, 20 µM Incubation Time: 48 hours Result: Induced apoptosis of NSCLC cell lines. Western Blot Analysis Cell Line: A549 and H460 cancer cell lines Concentration: 10 µM, 20 µM Incubation Time: 48 hours Result: Decreased survivin, c-IAP-1 and c-IAP-2 expression.

Cell Line:

A549 and H460 cancer cell lines

Concentration:

10 µM, 20 µM, 40µM

Incubation Time:

48 hours

Result:

Inhibited the proliferation of NSCLC cell lines.

Cell Line:

A549 and H460 cancer cell lines

Concentration:

10 µM, 20 µM

Incubation Time:

48 hours

Result:

Induced apoptosis of NSCLC cell lines.

Cell Line:

A549 and H460 cancer cell lines

Concentration:

10 µM, 20 µM

Incubation Time:

48 hours

Result:

Decreased survivin, c-IAP-1 and c-IAP-2 expression.