野菊花内酯

中文名称:

野菊花内酯

中文同义词:

野菊花内酯;野菊花内酯,分析标准品;野菊花内酯标准品;野菊花内酯对照品

英文名称:

handelin

英文同义词:

HANDELIN; YEJUHUALACTONE;handelin;Yejuhualactone;6,6-dihydroxy-6,6,9,9-tetramethyl-3-methylene-2,2-dioxo-2,3a,3,3a,4,5,5,6,6a,6,7,9a,9b,9,9a,9b-hexadecahydro-4H-spiro[azuleno[4,5-b]furan-3,10-[6a,9]ethanoazuleno[4,5-b]furan]-5-yl acetate;Spiro[azuleno[4,5-b]furan-3(2H),10-[4H-6a,9]ethanoazuleno[4,5-b]furan]-2,2(3H)-dione, 4-(acetyloxy)-3a,3a,4,5,5,6,6,6a,7,9,9a,9a,9b,9b-tetradecahydro-6,6-dihydroxy-6,6,9,9-tetramethyl-3-methylene-, (3R,3aR,3aS,4S,6R,6R,6aR,6aR,9R,9aR,9aS,9bR,9bS)-

CAS号:

62687-22-3

分子式:

C32H40O8

分子量:

552.66

EINECS号:

相关类别:

中药对照品;植物提取物;标准品;分析试剂标准品;对照品;对照品-中药对照品;标准品 -中药标准品;对照品

Mol文件:

62687-22-3.mol

沸点

731.0±60.0 °C(Predicted)

密度

1.34±0.1 g/cm3 (20 ºC 760 Torr)

酸度系数(pKa)

14.34±0.70(Predicted)

生物活性

Handelin 是来自Chrysanthemum boreale 的愈创木酚内酯二聚体，它通过下调 NF-κB 信号传导和促炎性细胞因子的产生而具有强大的抗炎活性。

体外研究

Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment suppresses the LPS-induced (1 μg/mL) overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Handelin also suppresseS the induction of pro-inflammatory cytokines TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells. Handelin also suppresses the activation of mitogen-activated protein kinases, including ERK and JNK signaling. Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment significantly reduces the iNOS and COX-2 mRNA levels in LPS- stimulated RAW 264.7 cells. The transcriptional activity of NF-κB stimulated with LPS is also suppressed by Handelin. In addition, the LPS-stimulated upregulation of miRNA-155 expression is suppressed by Handelin. RT-PCR Cell Line: RAW 264.7 cells Concentration: 10 μM, 20 μM, 40 μM Incubation Time: 5 hours Result: iNOS and COX-2 mRNA levels were significantly reduced. The transcriptional activity of NF-κB stimulated with LPS was also suppressed and the LPS-stimulated upregulation of miRNA-155 expression was also suppressed. Western Blot Analysis Cell Line: RAW 264.7 cells Concentration: 10 μM, 20 μM, 40 μM Incubation Time: Result: Suppressed the LPS-induced overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Suppressed the induction of pro-inflammatory cytokines TNF-α and IL-1β, and also also suppressed the activation of mitogen-activated protein kinases, including ERK and JNK signaling in LPS-stimulated RAW 264.7 cells.

Cell Line:

RAW 264.7 cells

Concentration:

10 μM, 20 μM, 40 μM

Incubation Time:

5 hours

Result:

iNOS and COX-2 mRNA levels were significantly reduced. The transcriptional activity of NF-κB stimulated with LPS was also suppressed and the LPS-stimulated upregulation of miRNA-155 expression was also suppressed.

Cell Line:

RAW 264.7 cells

Concentration:

10 μM, 20 μM, 40 μM

Incubation Time:

Result:

Suppressed the LPS-induced overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Suppressed the induction of pro-inflammatory cytokines TNF-α and IL-1β, and also also suppressed the activation of mitogen-activated protein kinases, including ERK and JNK signaling in LPS-stimulated RAW 264.7 cells.

体内研究

Handelin (Compound 1; 10-20 mg/kg; oral administration; for 30 mintues; male Sprague-Dawley rats) treatment inhibits acute inflammation in carrageenan-induced paw edema model. The serum level of IL-1β is also inhibited by Handelin in a carrageenan-induced paw edema model . Animal Model: Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan Dosage: 10 mg/kg, 20 mg/kg Administration: Oral administration; for 30 mintues Result: Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.

Animal Model:

Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan

Dosage:

10 mg/kg, 20 mg/kg

Administration:

Oral administration; for 30 mintues

Result:

Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.

化学性质

白色粉末，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于野菊花。

用途

野菊花内酯具有清热解毒，消肿，降压的作用。