中文名称:
WNT AGONIST 1 (CID11210285 盐酸盐)
中文同义词:
WNT AGONIST 1 CID11210285 盐酸盐 BML-284;N4-(苯并[D][1,3]二氧杂环戊烯-5-基甲基)-6-(3-甲氧基苯基)嘧啶-2,4-二胺;WNT AGONIST 1 (CID11210285 盐酸盐);2-氨基-4-[[3,4-(亚甲二氧基)苄基]氨基]-6-(3-甲氧基苯基)嘧啶;WNT信号激活剂(BML-284);化合物WNT AGONIST 1
英文同义词:
AMBMP;CID 11210285 hydrochloride;N4-(1,3-benzodioxol-5-ylmethyl)-6-(3-methoxyphenyl)-2,4-pyrimidinediamine hydrochloride;Wnt Agonist;Wnt agonist 1;BML-284;2-Amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine hydrochloride;Wnt agonist 1 (BML-284)
沸点
623.4±65.0 °C(Predicted)
密度
1.353±0.06 g/cm3(Predicted)
溶解度
DMSO: soluble20mg/mL, clear
酸度系数(pKa)
6.62±0.10(Predicted)
生物活性
BML-284 是一种有效的细胞通透性 Wnt 信号激活剂。BML-284 诱导 TCF 依赖的转录活性，其 EC50>700 nM 。
靶点
EC50: 700 nM (TCF-dependent transcriptional activity)
体外研究
BML-284 (10 µM; 24 hours) significantly increases the migration and invasion of both MNK45 and AGS cells and partially restores the migratory and invasive abilities of cells inhibited by pizotifen.BML-284 (10 µM; 24 hours) induces the expression of β-catenin significantly when compared with the NC group. It also partially reverses the effects induced by pizotifen on E-cadherin and N-cadherin expression in MNK45 and AGS cells compared with the pizotifen-treated group.
Western Blot Analysis
Cell Line: Human gastric cancer cell lines MNK45 and AGS [1]
Concentration: 10 µM
Incubation Time: 24 hours
Result: Induced β-catenin expression and reserved E-cadherin and N-cadherin expression in MNK45 and AGS cells.
Cell Line:
Human gastric cancer cell lines MNK45 and AGS [1]
Result:
Induced β-catenin expression and reserved E-cadherin and N-cadherin expression in MNK45 and AGS cells.
体内研究
BML-284 (10 ng) combines with Pyrimethanil (4 mg/L) could partially rescue the malformed phenotype and cardiac defects induced by Pyrimethanil in Tg (myl7:EGFP) transgenic embryos at 5.5 hpf are transferred into plates with 20 embryos.
