5,5-二硫双(2-硝基苯甲酸)

中文名称:

5,5-二硫双(2-硝基苯甲酸)

中文同义词:

5,5-二硫双(2-硝基苯甲酸);二硫代硝基苯甲酸;5,5-二硫代-双(硝基苯甲酸);5,5＇二硫代双(2-硝基苯甲酸);6,6ˊ-二硝基-3,3ˊ-二硫代苯甲酸;双(3-羧基-4-硝基苯基)二硫;5,5-二硫代二(2-硝基苯甲酸);5,5-二巯基(2-硝基苯甲酸)

英文名称:

5,5′-Dithiobis(2-nitrobenzoic acid)

英文同义词:

DTNB;ellmanns reagent;ELLMANS REAGENT;ELLMANS REAGENT;BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE;3-CARBOXY-4-NITROPHENYL DISULFIDE;Ellmsnsreagent;Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans

CAS号:

69-78-3

分子式:

C14H8N2O8S2

分子量:

396.35

EINECS号:

200-714-4

相关类别:

生化试剂;检测，显色，标记试剂;生物化学;细胞生物;化学试剂;羧酸;芳烃;胆固醇显色剂系列;检测标志试剂;Phenyls Phenyl-Het;Phenyls Phenyl-Het;有机砌块;色素类;marker;通用试剂;其他生化试剂;生化试剂-色素类;合成中间体;生物试剂;化工原料;医药原料;分析试剂标准品

Mol文件:

69-78-3.mol

熔点 

240-245 °C (dec.)(lit.)

沸点 

671.9±55.0 °C(Predicted)

密度 

1.67

折射率 

1.6900 (estimate)

储存条件 

Keep in dark place,Sealed in dry,Room Temperature

溶解度 

Do you have solubility information on this product that you would like to share

酸度系数(pKa)

1.57±0.25(Predicted)

形态

Yellow solid

水溶解性 

Soluble in water and ethanol.

Merck 

14,3551

BRN 

1896821

稳定性

Stable. Incompatible with strong bases, strong oxidizing agents.

InChIKey

KIUMMUBSPKGMOY-UHFFFAOYSA-N

CAS 数据库

69-78-3(CAS DataBase Reference)

EPA化学物质信息

Benzoic acid, 3,3-dithiobis[6-nitro- (69-78-3)

生物活性

DTNB (Ellman’s Reag, Ellmans Reagenz, 5,5′-Dithiobis(2-nitrobenzoic acid), 5,5′-Dithiobis-2-nitrobenzoesäure)是一种非荧光探针，可用于定量样品中巯基的数量或浓度。DTNB 还是登革热病毒蛋白酶 protease (NS2B-NS3pro)和链霉菌蛋白酶的变构抑制剂。

体外研究

DTNB reacts with the free sulfhydryl side chain of cysteine to form an S-S bond between the protein and a thionitrobenzoic acid (TNB) residue. The main advantage of DTNB over alternative reagents (e.g., N-ethylmaleimide or iodoacetamide) is in the selectivity of this reagent and in the ability to follow the course of the reaction spectrophotometrically. Modification of Enzyme with DTNB: Modification of the SH groups of the enzyme is carried out by reacting 10 μL of 10 mM DTNB solution (about a 20-fold molar excess) at room temperature with 0.6 mL of enzyme solution (0.807 mg/mL) in 80 mM phosphate buffer, pH 8.0, containing 1 mM EDTA, which has been dialyzed previously against the same buffer solution for 24 h. The number of SH groups is estimated from the increase of absorbance at 410 nm using a molar extinction coefficient of 13,600 M -1 cm -1 for thionitrobenzoate ions liberated.

用途 

广泛用于生化研究，是生化反应色原，诊断试剂研究生产，测定蛋白质、多肽和组织中巯基的灵敏测定试剂。有机磷农药中毒的监测（胆碱酯酶测定）。

用途 

用于测定蛋白质、多肽和组织中巯基的灵敏试剂，有机磷农药中毒的监测(胆碱酯酶测定)

用途 

巯基测定，是测定蛋白质、多肽和组织中巯基的灵敏试剂。也用于有机磷农药中毒的监测（胆碱酯酶测定）。

危险品标志 

Xi

危险类别码 

36/37/38

安全说明 

26-36

WGK Germany 

3

RTECS号

DG9650000

F 

10

TSCA 

Yes

海关编码 

29309070