延命草素

中文名称:

延命草素

中文同义词:

延命草素;延命素;延命素,延命草素

英文名称:

enmein

英文同义词:

enmein;Isodonin

CAS号:

3776-39-4

分子式:

C20H26O6

分子量:

362.42

EINECS号:

相关类别:

中药对照品;对照品;二萜;标准品 -中药标准品

Mol文件:

3776-39-4.mol

熔点 

274-276℃ (Decomposition) (ethanol )

沸点 

610.3±55.0 °C(Predicted)

密度 

1.37±0.1 g/cm3(Predicted)

酸度系数(pKa)

12.66±0.60(Predicted)

生物活性

Enmein 从 I. serra 中分离，具有免疫抑制作用。

体外研究

Enmein shows significant inhibitory effect toward K562 with an IC 50 value ranging from 3.2 μg/ml to 8.2 μg/ml. Enmein (3.13-25 μg/ml; 48 hours) has inhibitory effect on lymphocytes with significant difference within concentration from 6.25 μg/ml to 25 μg/ml. Enmein (5.5-22 μg/ml; 48 hours) causes the percentage of S stage cells decreased from 19% to 6%, causes the percentage of G2/M stage cells to decrease from 2% to 0.4%. Cell Viability Assay Cell Line: Splenic lymphocyte cells Concentration: 3.13, 6.25, 12.5, 25 μg/ml Incubation Time: 48 hours Result: Exhibited an IC 50 value of 17 μg/ml in splenic lymphocytes. Cell Cycle Analysis Cell Line: Splenic lymphocyte cells Concentration: 5.5 μg/ml, 11 μg/ml, 22 μg/ml Incubation Time: 48 hours Result: Caused S stage cells and G2/M stage cells decreased.

Cell Line:

Splenic lymphocyte cells

Concentration:

3.13, 6.25, 12.5, 25 μg/ml

Incubation Time:

48 hours

Result:

Exhibited an IC 50 value of 17 μg/ml in splenic lymphocytes.

Cell Line:

Splenic lymphocyte cells

Concentration:

5.5 μg/ml, 11 μg/ml, 22 μg/ml

Incubation Time:

48 hours

Result:

Caused S stage cells and G2/M stage cells decreased.

体内研究

Enmein (inject to ear; 6.52-13.04 mg/kg; 5 days) inhibits the xylene-induced ear swelling and inhibits IL-2 expression. Animal Model: Balb/c mice Dosage: 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg Administration: Injected to ear; 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg; 5 days Result: Had inhibitory effect on IL-2 release in blood.

Animal Model:

Balb/c mice

Dosage:

6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg

Administration:

Injected to ear; 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg; 5 days

Result:

Had inhibitory effect on IL-2 release in blood.

化学性质 

白色针状色结晶，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于溪黄草。