黄柏苷

中文名称:

黄柏苷

中文同义词:

二氢黄柏苷;黄柏甙;(二氢)黄柏甙;黄柏苷

英文名称:

phellamurin

英文同义词:

phellamurin;(2R)-7-(β-D-Glucopyranosyloxy)-2,3-dihydro-3β,5-dihydroxy-2α-(4-hydroxyphenyl)-8-(3-methyl-2-butenyl)-4H-1-benzopyran-4-one;(dihydro) Phellodendron;4H-1-Benzopyran-4-one,7-(b-D-glucopyranosyloxy)-2,3-dihydro-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-,(2R,3R)-;4H-1-Benzopyran-4-one, 7-(β-D-glucopyranosyloxy)-2,3-dihydro-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-, (2R,3R)-

CAS号:

52589-11-4

分子式:

C26H30O11

分子量:

518.52

EINECS号:

相关类别:

标准品;植物提取物;标准品 -对照药材;黄酮

Mol文件:

52589-11-4.mol

沸点 

861.5±65.0 °C(Predicted)

密度 

1.504±0.06 g/cm3(Predicted)

酸度系数(pKa)

7.15±0.60(Predicted)

生物活性

Phellamurin 是一种来自 Phellodendron amurense 叶的植物黄酮糖苷，可抑制肠道 P-糖蛋白 (P-glycoprotein)。Phellamurin 还抑制凤蝶的产卵。Phellamurin 诱导细胞凋亡 (apoptosis)，并具有抗肿瘤活性。

体外研究

Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment leads to a repression of cell viability in U2OS and Saos-2 cells in a dose-dependent manner. Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment concentration-dependently promots the apoptosis of U2OS and Saos-2 cells. Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment declines the ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR in U2OS and Saos-2 cells. Cell Viability Assay Cell Line: U2OS and Saos-2 cells Concentration: 0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL Incubation Time: 48 hours Result: Suppressed the viability of U2OS and Saos-2 cells in a concentration-dependent manner. Apoptosis Analysis Cell Line: U2OS and Saos-2 cells Concentration: 0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL Incubation Time: 48 hours Result: Induced apoptosis of U2OS and Saos-2 cells in a concentration-dependent manner. Western Blot Analysis Cell Line: U2OS and Saos-2 cells Concentration: 0 μg/mL and 10 μg/mL Incubation Time: 48 hours Result: Repressed the PI3K/AKT/mTOR pathway in U2OS and Saos-2 cells.

Cell Line:

U2OS and Saos-2 cells

Concentration:

0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL

Incubation Time:

48 hours

Result:

Suppressed the viability of U2OS and Saos-2 cells in a concentration-dependent manner.

Cell Line:

U2OS and Saos-2 cells

Concentration:

0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL

Incubation Time:

48 hours

Result:

Induced apoptosis of U2OS and Saos-2 cells in a concentration-dependent manner.

Cell Line:

U2OS and Saos-2 cells

Concentration:

0 μg/mL and 10 μg/mL

Incubation Time:

48 hours

Result:

Repressed the PI3K/AKT/mTOR pathway in U2OS and Saos-2 cells.

体内研究

Phellamurin (50 mg/kg/day; intraperitoneal injection; daily; for 21 days; female BALB/c nude mice) treatment represses osteosarcoma tumor growth in vivo. The ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR are decreased in xenograft in Phellamurin-treated mice. Animal Model: Athymic female BALB/c nude mice (6 weeks old ) injected with U2OS cells Dosage: 50 mg/kg/day Administration: Intraperitoneal injection; daily; for 21 days Result: Repressed osteosarcoma tumor growth in vivo.

Animal Model:

Athymic female BALB/c nude mice (6 weeks old ) injected with U2OS cells

Dosage:

50 mg/kg/day

Administration:

Intraperitoneal injection; daily; for 21 days

Result:

Repressed osteosarcoma tumor growth in vivo.