BETA-淀粉样蛋白片断25-35

中文名称:

BETA-淀粉样蛋白片断25-35

中文同义词:

BETA-淀粉样蛋白片断25-35;淀粉样Β-蛋白片段25-35;AMYLOID Β-PROTEIN FRAGMENT 25-35 Β-淀粉状蛋白25-35;BETA-类淀粉蛋白肽(25-35);Β-淀粉状蛋白25-35;Β-AMYLOID (25-35);Β-淀粉状蛋白25-35;Β淀粉样蛋白25-35

英文名称:

AMYLOID BETA-PROTEIN (HUMAN, 25-35) TRIFLUOROACETATE

英文同义词:

Amyloid beta-peptide(25-35);AMYLOID BETA-PROTEIN (HUMAN, 25-35) TRIFLUOROACETATE;H-GLY-SER-ASN-LYS-GLY-ALA-ILE-ILE-GLY-LEU-MET-OH;AMYLOID BETA-PEPTIDE (25-35) (HUMAN);AMYLOID BETA-PROTEIN FRAGMENT 25-35;AMYLOID BETA-PROTEIN (25-35);AMYLOID-BETA PROTEIN (25-35), HUMAN, MOUSE, RAT;Amyloid beta-Protein (25-35) trifluoroacetate salt

CAS号:

131602-53-4

分子式:

C45H81N13O14S

分子量:

1060.27

EINECS号:

相关类别:

细胞生物学;多肽;细胞信号和神经生物学;多肽，氨基酸;其他生化试剂;生化试剂-蛋白质;目录肽;Peptide;Alzheimers and Neurodegenerative Disease Research;Amyloid beta Protein Fragments;Amyloid β Protein FragmentsNeuropeptides;Neurodegenerative Disease Peptides;β Amyloid Peptides;Amyloid beta-peptide and related;Alzheimer;proteins

Mol文件:

131602-53-4.mol

沸点 

1517.3±65.0 °C(Predicted)

密度 

1.248±0.06 g/cm3(Predicted)

RTECS号

BU7435200

储存条件 

-20°C

酸度系数(pKa)

3.24±0.10(Predicted)

形态

Lyophilized.

水溶解性 

Soluble in water.

InChIKey

WIHBNMPFWRHGDF-SLVFWPMISA-N

生物活性

β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) 是阿尔茨海默氏淀粉样蛋白β肽的Aβ(25-35) 片段，在培养细胞中显示出神经毒性活性。

体外研究

The amino acid sequence of Aβ(25-35) peptide is NH 2 -Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy. Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events. β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure. 2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃. 3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing. 4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments. Methods vary depends on the downstream applications.

体内研究

Rats are injected with Aβ25–35 peptide intracerebroventricularly and compound Danshen (CDS) are subsequently administered once daily for 23 days. Rats’ behavior is monitored using Morris water maze and passive avoidance. Real time PCR and Western blotting are used in determining amyloid precursor protein (APP), β-site APP cleaved enzyme-1(BACE1), Presenilin-1 (PS1), Insulin-degrading enzyme (IDE) and neprilysin (NEP) in hippocampus. The Alzheimer s disease (AD) model group present with spatial learning and memory impairments. CDS and donepezil administration significantly ameliorate the Aβ25–35 peptide-induced memory impairment in both Morris water maze (P [3] .

用途 

Amyloid β的功能区。兼具有神经营养和神经毒素效果。用于分子生物学研究。

WGK Germany 

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