荧光专用试剂

中文名称:

荧光专用试剂

中文同义词:

MBBR[单溴二胺];单溴代双满;溴代双满;溴二胺;荧光专用试剂;溴代双满,溴二胺,MONOBROMOBIMANE(MBBR );单溴二胺 MBBR

英文名称:

MONOBROMOBIMANE

英文同义词:

MBBR;MONOBROMOBIMANE;MMB;THIOLYTE MONOBROMOBIMANE REAGENT;THIOLYTE(R) MB;THIOLYTE(R) MB REAGENT;THIOLYTE(R) MONOBROMOBIMANE REAGENT;THIOLYTE(R) MONOBROMOBIMANE REAGENT, FLUORESCENT LABELING COMPOUND

CAS号:

71418-44-5

分子式:

C10H11BrN2O2

分子量:

271.11

EINECS号:

相关类别:

荧光探针，标记，颗粒和染色;基准试剂;Pyrazole;Fluorescent Labels and Indicators;Fluorescent Labels Indicators;MTS Sulfhydryl Active Reagents

Mol文件:

71418-44-5.mol

熔点 

152-154 °C(lit.)

沸点 

327.8±44.0 °C(Predicted)

密度 

1.66±0.1 g/cm3(Predicted)

储存条件 

2-8°C

溶解度 

DMF: soluble

形态

powder

酸度系数(pKa)

-3.36±0.70(Predicted)

颜色

Yellow

最大波长(λmax)

398 nm

BRN 

4430959

稳定性

Light Sensitive

生物领域应用

Glutathione S-transferase substrates; detecting glutathione S-transferase,thiols,sulfite,homocysteine,mycothiol,sulfur compounds; thiol-reactive probes

CAS 数据库

71418-44-5

生物活性

Bromobimane基本上是非荧光物质，当与小硫醇反应时，生成荧光产物。

体外研究

Bromobimanes in solution (aqueous or organic solvents of medium polarity) react with small thiols [e.g., the tripeptide thiol glutathione (GSH)], and with reactive protein thiol groups (e.g., hemoglobin). The reactions of bromobimanes with thiols are second order and dependent on pH, the active nucleophile being the thiolate anion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-soluble fluorescent products. The neutral agents mBBr and bBBr are moderately soluble in mediumpolarity organic solvents (acetonitrile, dichloromethane), and slightly soluble in water. The quaternary salt, qBBr, and the anionic bromobimane, SBBr, are soluble in water, but less soluble in organic solvents. Bromobimanes are yellow. The highly selective, rapid reactivity of bromobimanes toward thiols, the stability and fluorescence yield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and the availability of both cell-penetrating and nonpenetrating forms, make the use bromobimanes an extremely powerful approach to the analysis of low molecular weight biothiols.

安全说明 

22-24/25

WGK Germany 

3

F 

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