5-羧基荧光素二乙酸酯

中文名称:

5-羧基荧光素二乙酸酯

中文同义词:

5-羧基荧光素二醋酸盐;5-羧基荧光素双醋酸盐;5-羧基荧光素二乙酸酯;5-羧基荧光素 二醋酸;5-CARBOXYFLUORESCEIN DIACETATE 5-羧基荧光素二醋酸盐

英文名称:

5-CARBOXYFLUORESCEIN DIACETATE

英文同义词:

5-Carboxy-di-O-acetylfluorescein, 5-CFDA;5-FAM DA;3,6-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9-[9H]xanthene]-5-carboxylic Acid;5-Carboxyfluorescein diacetate;3,6-Diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9-xanthene]-5-carboxylic acid;5-CFDA [5-Carboxyfluorescein diacetate];5-Carboxyfluorescein diacetate≥ 95% (HPLC);BIOCHEMIKA, FOR FLUORESCENCE, ≥95.0% (HPLC)

CAS号:

79955-27-4

分子式:

C25H16O9

分子量:

460.39

EINECS号:

相关类别:

血液学和组织学;染色剂和染料;其它;marker;Aromatics;Heterocycles;Intermediates Fine Chemicals;Pharmaceuticals;Fluorescent;Xanthene

Mol文件:

79955-27-4.mol

熔点 

>250°C

沸点 

693.1±55.0 °C(Predicted)

密度 

1.57±0.1 g/cm3(Predicted)

储存条件 

Sealed in dry,Store in freezer, under -20°C

溶解度 

DMSO: soluble

酸度系数(pKa)

6.4(at 25℃)

形态

Solid

颜色

Colorless solid

酸碱指示剂变色ph值范围

Weak green uorescence (6.0) to strong green uourescence (7.2)

最大波长(λmax)

<300nm

BRN 

68265

生物领域应用

Analyzing intracellular glutathione levels in cells; assessing gap junctional intercellular communication in cells; characterizing antifungal activity of micafungin, caspofungin; counting/detecting/evaluating microorganisms; detecting cells; detecting/measuring intracellular pH in yeast; determining extracellular/intracellular pH in bacteria under CO2 treatment; evaluating/measuring keratinocytes growth rate; identifying cancerous cells measuring gas (carbon dioxide or ammonia) concentration in body fluids; screening xenobiotic phloem mobility in plants; studying low-density lipoprotein (LDL)/cholesterol metabolism; as a substrate for measuring esterase activity

主要应用

Sensors, method for measuring bacteria, detecting intracellular pH inyeast, determination of extracellular and intracellular pH of microorganisms, detecting living cells, identification of cancerous cells, method for assessing microorganism viability, proliferative activities in tumor tissues, measuring the growth of keratinocytes, agent, neurodegenerative disorders, antineoplastic drugs

生物活性

5-CFDA(5-Carboxyfluorescein diacetate)是一种可渗入细胞并被水解为5-carboxyfluorescein，用于标记人椎间盘细胞以便体外荧光显微镜观察。

用途 

一种重要的染色剂,渗透完整细胞膜的非荧光疏水剂。在细胞中，5- 羧基荧光素二乙酸酯被酶水解成亲水剂。

危险品标志 

T

危险类别码 

45-46-23/24/25-36/37/38

安全说明 

53-22-26-36/37/39-45

WGK Germany 

3

F 

10-21

海关编码 

32049010