灵芝酸D

中文名称:

灵芝酸D

中文同义词:

灵芝酸D;灵芝酸D对照品,;灵芝酸D标准品

英文名称:

Ganoderic acid D

英文同义词:

(7beta)-7-Hydroxy-3,11,15,23-tetraoxolanost-8-en-26-oic acid;Lanost-8-en-26-oic acid, 7-hydroxy-3,11,15,23-tetraoxo-, (7β)-

CAS号:

108340-60-9

分子式:

C30H42O7

分子量:

514.65

EINECS号:

相关类别:

三萜;植物提取物;对照品，标准品;中药对照品;标准品;对照品;对照品-中药对照品;chemical reagent;pharmaceutical intermediate;phytochemical;reference standards from Chinese medicinal herbs (TCM).;standardized herbal extract;标准品 -中药标准品;标准品，对照品;标准品-对照品

Mol文件:

108340-60-9.mol

沸点 

688.3±55.0 °C(Predicted)

密度 

1.22±0.1 g/cm3(Predicted)

酸度系数(pKa)

4.77±0.23(Predicted)

生物活性

Ganoderic acid D 是一种高度氧化的四环三萜类化合物 (tetracyclic triterpenoid)，是灵芝 (Ganoderma lucidum) 的主要活性成分。Ganoderic acid D 上调 SIRT3 的蛋白质表达并通过 SIRT3 诱导脱乙酰化的亲环蛋白 D (CypD)。Ganoderic acid D 抑制结肠癌细胞的能量重编程，包括结肠癌细胞中的葡萄糖摄取，乳酸产生，丙酮酸和乙酰辅酶的产生。Ganoderic acid D 诱导 HeLa 人宫颈癌细胞凋亡 (apoptosis)。

靶点

SIRT3

SIRT3

体外研究

Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC 50 of 17.3 mM. Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner. Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest. Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells. Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3. Cell Viability Assay Cell Line: HeLa human cervical carcinoma cell line (CCL-2) Concentration: 1, 5, 10, 20, 50 μM Incubation Time: 24, 48, 72 hours Result: Reduced the cell survival rate in a dose- and time-dependent manner and had an IC 50 value of 17.3 μM for 48 hours treatment. Cell Cycle Analysis Cell Line: HeLa human cervical carcinoma cell line (CCL-2) Concentration: 10, 50 μM Incubation Time: 24, 48 hours Result: Induced G2/M phase arrest. Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM. Apoptosis Analysis Cell Line: HeLa human cervical carcinoma cell line (CCL-2) Concentration: 10, 50 μM Incubation Time: 48 hours Result: Induced a morphological change typical of apoptosis in HeLa cells. Western Blot Analysis Cell Line: HeLa human cervical carcinoma cell line (CCL-2) Concentration: 10 μM Incubation Time: 48 hours Result: Up-regulated 14-3-3E and PRDX3.

Cell Line:

HeLa human cervical carcinoma cell line (CCL-2)

Concentration:

1, 5, 10, 20, 50 μM

Incubation Time:

24, 48, 72 hours

Result:

Reduced the cell survival rate in a dose- and time-dependent manner and had an IC 50 value of 17.3 μM for 48 hours treatment.

Cell Line:

HeLa human cervical carcinoma cell line (CCL-2)

Concentration:

10, 50 μM

Incubation Time:

24, 48 hours

Result:

Induced G2/M phase arrest. Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM.

Cell Line:

HeLa human cervical carcinoma cell line (CCL-2)

Concentration:

10, 50 μM

Incubation Time:

48 hours

Result:

Induced a morphological change typical of apoptosis in HeLa cells.

Cell Line:

HeLa human cervical carcinoma cell line (CCL-2)

Concentration:

10 μM

Incubation Time:

48 hours

Result:

Up-regulated 14-3-3E and PRDX3.

化学性质 

白色粉末，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于多孔菌科灵芝Ganodermalucidum（Leyss.exFr.）Karst．的干燥子实体。

用途 

灵芝酸D具有降血脂、降血压的作用。

用途 

用于含量测定/鉴定/药理实验等 药理药效:抗肿瘤，保肝，心血管方面作用