双链聚肌胞
中文名称:
双链聚肌胞
中文同义词:
聚肌苷酸-聚胞苷酸钠盐;双链聚肌胞;6.双链聚肌胞(POLYI:C .);卡那霉素-聚肌胞钠盐复合物;双链聚肌胞钠盐;双链聚肌胞溶液;POLYI:C双链聚肌胞钠盐;聚肌苷 - 聚胞苷酸钠盐
英文名称:
POLYINOSINIC-POLYCYTIDYLIC ACID SODIUM SALT
英文同义词:
POLYINOSINIC ACID + POLYCYTIDYLIC ACID, SODIUM SALT;Polymyocyte sodium salt;POLYINOSINIC ACID POLYCYTIDYLIC ACID, SODIUM SALT;POLYINOSINIC-POLYCYTIDYLIC ACID SODIUM SALT;POLY I POLY C, NA;POLY[I] POLY[C] SODIUM SALT;Poly(I) •;Oligochitosan-kanamycin-polyI-polyC sodium salt compound solution
CAS号:
42424-50-0
分子式:
C19H27N7O16P2
分子量:
671.41
EINECS号:
1308068-626-2
相关类别:
医药中间体;中间体;兽用鉰料添加剂和婴幼儿奶粉添加;医药兽药;原料药;生化试剂-聚合物类;Biochemicals and Reagents;Biomaterials;Cell Culture;Materials Science;Nucleosides;Nucleotides;Oligonucleotide Polymers and Copolymers;Oligonucleotides;Other adjuvants;Synthetic Oligonucleotides and Related Reagents;Vaccine Adjuvants;Vaccine Production
Mol文件:
42424-50-0.mol
储存条件
−20°C
溶解度
H2O: soluble10mg/mL
形态
lyophilized powder
生物活性
Polyinosinic-polycytidylic acid sodium (Poly(I:C) sodium) 是双链 RNA 的合成类似物，是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Polyinosinic-polycytidylic acid sodium 可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡。
靶点
TLR3, RIG-I, MDA5, apoptosis
体外研究
Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours.
Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells.
Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1.
Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells.
Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use.
Cell Viability Assay
Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
Concentration: 20 ng/mL
Incubation Time: 24 hours
Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.
Apoptosis Analysis
Cell Line: 1205Lu cells
Concentration: 200 ng/mL
Incubation Time: 24 hours
Result: Induced apoptosis in 1205Lu cells.
RT-PCR
Cell Line: 1205Lu cells
Concentration: 3 ng/mL
Incubation Time: 24 hours
Result: Induced IFN-β expression in melanoma cells.
Western Blot Analysis
Cell Line: 1205Lu cells
Concentration: 5 ng/mL
Incubation Time: 24 hours
Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
Cell Line:
WM793, WM278, WM239A, WM9 and 1205Lu cells
Concentration:
20 ng/mL
Incubation Time:
24 hours
Result:
Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.
Cell Line:
1205Lu cells
Concentration:
200 ng/mL
Incubation Time:
24 hours
Result:
Induced apoptosis in 1205Lu cells.
Cell Line:
1205Lu cells
Concentration:
3 ng/mL
Incubation Time:
24 hours
Result:
Induced IFN-β expression in melanoma cells.
Cell Line:
1205Lu cells
Concentration:
5 ng/mL
Incubation Time:
24 hours
Result:
Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
体内研究
Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid.
用途
一种可作为在TLR3水平对细胞信号转导进行研究的模型RNA的双链均聚物。也是一种TRIF依赖性toll样受体-3(TLR3)配体。
安全说明
22-24/25
WGK Germany
3
RTECS号
TR0175000
