D-荧光素钾盐

中文名称:

D-荧光素钾盐

中文同义词:

(S)-4,5-二氢-2-(6-羟基苯并噻唑-2-基)噻唑-4-甲酸钾盐;D-荧光素钾盐, ≥98.0% (HPLC);D-荧光素钾盐POTASSIUM (S)-2-(6-HYDROXYBENZO[D]THIAZOL-2-YL)-4,5-DIHYDROTHIAZOLE-4-CARBOXYLATE;D-LUCIFERIN POTASSIUM SALT D-虫荧光素钾盐;D-荧光素钾、D-冷光素钾;D-LUCIFERIN POTASSIUM SALT 98% 荧光素钾盐;D-荧光素钾;荧光素钾

英文名称:

D-LUCIFERIN POTASSIUM SALT

英文同义词:

D-(-)-2-[6-HYDROXY-2-BENZOTHIAZOLYL]DELTA-THIAZOLINE-4-CARBOXYLIC ACID POTASSIUM SALT;D-LUCIFERIN FIREFLY, POTASSIUM SALT;D-LUCIFERIN, MONOPOTASSIUM SALT;D-LUCIFERIN POTASSIUM SALT;BEETLE LUCIFERIN, POTASSIUM SALT;4,5-DIHYDRO-2-(6-HYDROXY-2-BENZOTHIAZOLYL)-4-THIAZOLECARBOXYLIC ACID POTASSIUM SALT;D-LUCIFERIN K SALT;D -LUCIFERIN SYNTHETIC POTASSIUM SALT

CAS号:

115144-35-9

分子式:

C11H7KN2O3S2

分子量:

318.41

EINECS号:

1592732-453-0

相关类别:

医用原料;生物试剂;生化试剂;生物发光底物;发光化合物;医药中间体;化学发光;荧光染料;化学试剂;其他生化试剂;标准品 -中药标准品;医药原料;PCR试剂盒-活体成像用试剂系列;原料药;PCR试剂盒-荧光定量PCR试剂盒

Mol文件:

115144-35-9.mol

储存条件 

Inert atmosphere,Store in freezer, under -20°C

BRN 

5416262

InChIKey

UMBKGTQQGYPQBE-OGFXRTJISA-M

CAS 数据库

115144-35-9(CAS DataBase Reference)

简介

D-荧光素钾盐是荧光素酶的水溶性底物，存在于多种发光生物体中。在ATP和荧光素酶的催化作用下，D-荧光素钾被氧化，产生蓝绿色的光(560nm)，当底物过量时，产生的光量子数与荧光素酶的浓度呈正相关。编码荧光素酶的Luc基因是植物、哺乳动物细胞的常用报告基因。由于没有背景干扰，因此可以很容易地检测出低至0.02 pg 水平的荧光素酶。

用途

D-荧光素钾盐是一类在生物体中发现的能引起生物发光的杂环化合物，如萤火虫。在ATP存在下，萤光素酶将其氧化脱羧后会发光。化学研究中可用于荧光素酶的基板。

生物活性

D-Luciferin是萤火虫荧光素酶的底物。

体外研究

D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly Photinus pyralis , the enzyme more frequently used in techniques of in vitro and optical imaging. D-Luciferin exhibits a decrease in the measured K m in PC3M-Luc cell lysates with a K m of 34 μM.

体内研究

Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection. Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection. Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H 2 O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter.

用途 

活细胞、组织或生物体内luc标记基因和荧光素酶-融合基因体内/体外表达的成像分析； 2）广泛用于报告基因分析，免疫分析和ATP荧光卫生监测分析；

WGK Germany 

3

海关编码 

29342000