噻唑蓝

中文名称:

噻唑蓝

中文同义词:

噻唑蓝/3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝/噻唑兰/溴化噻唑蓝四氮唑/3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑鎓溴化物/2-(4,5-二甲基-2-噻唑基)-3,5-二苯基-2H-四唑氢溴酸盐;噻唑兰 250MG;3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝,噻唑兰,溴化噻唑蓝四氮唑;噻唑四唑蓝溴化盐;噻唑蓝;噻唑蓝溴化四唑,98%;3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四氮唑溴化物;溴化噻唑蓝四氮唑

英文名称:

Thiazolyl Blue

英文同义词:

2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazol-3-ium Bromide;Thiazolyl Blue Tetrazolium Bromide Vetec(TM) reagent grade, 98%;ACRYL/BIS 29:1 PREMIXED PWD ULTRA PURE;3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl;3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2,3-dihydro-1H-tetrazol-3-ium bromide;2-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-2,3-dihydro-1H-tetrazol-3-ium bromide;2,5-diphenyl-3-(4,5-dimethylthiazol-2-yl)-2h-tetrazolium Bromide;2,5-diphenyl-3-(4,5-dimethylthiazol-2-yl)tetrazolium Bromide

CAS号:

298-93-1

分子式:

C18H16BrN5S

分子量:

414.32

EINECS号:

206-069-5

相关类别:

其他生化试剂;细胞培养;色素类;生化试剂;对照品;细胞生物学试剂;分子生物试剂-细胞相关;蛋白质组学;基因组学和分子诊断;试剂和补充物;Tetrazolium salt;试剂;细胞分析;细胞相关;细胞增殖;生化试剂-色素类;生物化工;有机化工原料;化学试剂;化妆品添加剂-;医药原料;Tetrazolium Salts;Tetrazolium Salts Formazans;Chemistry;marker

Mol文件:

298-93-1.mol

熔点 

195 °C (dec.) (lit.)

密度 

1.4886 (estimate)

储存条件 

2-8°C

溶解度 

methanol: 5 mg/mL, clear

形态

powder

颜色

Yellow to orange

水溶解性 

Slightly soluble

最大波长(λmax)

378 nm, 242 nm

敏感性 

Light Sensitive

BRN 

3825277

生物领域应用

Cell viability assay; microbial growth assays; DNA quantification assays; tissue viability assays; detecting enzymes; measuring membrane potential; treating Alzheimer’s disease,asthma,cancer

InChIKey

AZKSAVLVSZKNRD-UHFFFAOYSA-M

CAS 数据库

298-93-1(CAS DataBase Reference)

EPA化学物质信息

2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (298-93-1)

生物活性

Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium)是一种细胞可渗透且带正电的比色剂，被广泛用于检测细胞中的还原代谢以及测量细胞增殖，细胞毒性和凋亡。Thiazolyl Blue (MTT) 在活细胞中会从黄色还原为紫色的甲臜。

体外研究

Thiazolyl Blue (MTT) is used in conjunction with rhodamine B to measure mitochondrial membrane potential. Thiazolyl Blue-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of Thiazolyl Blue is strong. Thiazolyl Blue is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis.

用途 

1．药物（也包括其他处理方式如放射线照射）对体外培养的细胞毒性的测定； 2．细胞增殖及细胞活性测定。

危险品标志 

Xi,T,Xn

危险类别码 

46-68-36/37/38

安全说明 

22-24/25-53-45-36/37-26

WGK Germany 

3

RTECS号

XF8060000

F 

3-8-10

TSCA 

Yes

海关编码 

29341000

毒害物质数据

298-93-1(Hazardous Substances Data)