(+)生物素-N-琥珀酰亚胺基酯

中文名称:

(+)生物素-N-琥珀酰亚胺基酯

中文同义词:

N-羟基琥珀酰亚胺 D-生物素;生物素-N-羟基丁二酰亚胺活化脂(BIOTIN-NHS);(+)生物素-N-琥珀酰亚胺基酯、N-羟基琥珀酰亚胺生物素;生物素琥珀酰亚胺基酯;NHS-BIOTIN 生物素-N-羟基丁二酰亚胺活化脂;(+)-生物素-N-琥珀酰亚胺基酯 250MG;琥珀酰胺生物素脂;(+)-生物素-N-羟基琥珀酰亚胺酯

英文名称:

BIOTIN-NHS

英文同义词:

(+)-biotin-n-hydroxysuccimide ester;n-hydroxysuccimidobiotin;BIOTIN-OSU: N-HYDROXYSUCCINIMIDOBIOTIN;d-Biotin NHS ester;(2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate;5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID 2,5-DIOXO-PYRROLIDIN-1-YL ESTER;D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER;D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER

CAS号:

35013-72-0

分子式:

C14H19N3O5S

分子量:

341.38

EINECS号:

609-055-0

相关类别:

氨基酸衍生化合物;生物素标记试剂;化学试剂;补充产品;医用原料;抗体;交联剂;Biotin Derivatives;Cross Linking Reagents;BiotinylationReagents;Biotinylation Reagents;biotinyl;标记物;生化试剂;其他生化试剂;生化试剂-维生素类;原料;PEG;其它

Mol文件:

35013-72-0.mol

熔点

212-214 °C

密度

1.45±0.1 g/cm3(Predicted)

储存条件

-20°C

溶解度

DMF: ≤50 mg/mL Stable for at least one month in dry DMF

酸度系数(pKa)

13.89±0.40(Predicted)

形态

powder

颜色

White to off-white

BRN

4720781

稳定性

Stable. Incompatible with strong oxidizing agents.

CAS 数据库

35013-72-0(CAS DataBase Reference)

简介

NHS生物素试剂可对溶液中的抗体，蛋白质和任何其他含伯胺的大分子进行简单有效的生物素标记。这三种试剂仅在间隔臂长度上有所不同，为研究人员提供了优化标记和检测实验的可能性，其中生物素结合的空间位阻是重要因素。

应用

生物活性

Biotin NHS是用于制备生物素化表面或多肽的氨基反应性生物素试剂。

体外研究

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Calculations : For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution. 1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess: mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin. * 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample 2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction: mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin Biotin Labeling Reaction : 1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations. 2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO. 3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution. 4. Incubate reaction on ice for two hours or at room temperature for 30 minutes. 5. To tests the labeled protein by ELISA or Western blot.

用途

可用于蛋白、抗原、抗体、核酸（DNA、RNA)等的标记

用途

可以用于蛋白质与肽的生物素酰化，如在pH6.5-8.5范围内偶联至伯胺

安全说明

22-24/25

WGK Germany

10-23

海关编码

2934 99 90

价格：请咨询卖家

CAS 号： 35013-72-0