中文名称:
4-(N-马来酰亚胺甲基)环己烷-1-羧酸磺酸基琥珀酰亚胺酯钠盐
中文同义词:
4-(N-马来酰亚胺甲基)环己烷-1-羧酸 3-磺酸基-N-羟基琥珀酰亚胺酯钠盐;4-(N-马来酰亚胺基甲基)环己烷-1-甲酸3-硫代-N-琥珀酰亚胺钠盐;磺基-SMCC钠盐;4-(马来酰亚胺基甲基)环己烷-1-羧酸 3-磺酸基-N-羟基琥珀酰亚胺酯钠盐, 98+%;硫代-SMCC 钠盐;4-(N-马来酰亚胺基甲基)环己烷-1-羧酸-3-硫代-N-琥珀酰亚胺酯钠盐;磺基琥珀酰亚胺基-4-(N-马来酰亚胺甲基)环己烷-1-羧酸酯;4-(N-马来酰亚胺甲基)环己烷-1-羧酸磺酸基琥珀酰亚胺酯钠盐(SULFO-SMCC)
英文同义词:
SULFO-N-SUCCINIMIDYL 4-(MALEIMIDOMETHYL)CYCLOHEXANE-1-CARBOXYLATE, SODIUM SALT;SULFOSUCCINIMIDYL-TRANS-4-(N-MALEIMIDOMETHYL)CYCLOHEXANE-1-CARBOXYLATE;SULFO-SMCC;SULFO SUCCINIMIDYL-4-(N-MALEIMIDO METHYL) CYCLOHEXANE-1-CARBOXYLATE;SMCC, WATER-SOLUBLE;4-(N-MALEIMIDOMETHYL)CYCLOHEXANE-1-CARBOXYLIC ACID 3-SULFO-N-HYDROXYSUCCINIMIDE ESTER SODIUM SALT;4-(N-maleimidomethyl)cyclohexane-1-*carboxylic ac;4-[(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)methyl]-Cyclohexanecarboxylic acid 2,5-dioxo-3-sulfo-1-pyrrolidinyl ester sodium salt (1:1)
相关类别:
其他生化试剂;蛋白异源双功能交链试剂;蛋白组学;交联剂;蛋白质结构分析;氨基酸衍生化合物;基准试剂;Maleimide Derivatives;Cross Linking Reagents;MTS Sulfhydryl Active Reagents;Protein Crosslinkers;Heterobifunctional Crosslinker;heteroXlink
储存条件
Inert atmosphere,Store in freezer, under -20°C
水溶解性
Soluble in water (2.5 mg/ml) and dimethyl formamide.
InChIKey
VUFNRPJNRFOTGK-UHFFFAOYSA-M
生物活性
Sulfo-SMCC sodium 是一种常用的异双官能交联剂，是不可降解的 ADC linker，其带有N-羟基琥珀酰亚胺 (NHS) 酯和马来酰亚胺基团，分别与伯胺和巯基反应。
体外研究
The crosslinker Sulfo-SMCC consists of a maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Sulfo-SMCC inhibits the end-to-end annealing of stabilized Microtubules (MTs). MTs are treated with 250 μM Sulfo-SMCC, and imaged after incubation for 0 h, 6 h, and 24 h. MTs treated with Sulfo-SMCC shows a constant mean length, independent of the incubation time.
用途
水溶性、双异官能团交联试剂，将胺以及巯基反应性合并入扩展间隔片，典型地，在pH7.5缓冲液(6.5-8.5)中，通过酰胺键偶联至包含伯胺的分子，在pH6.8缓冲液(6.5-7.0)中，通过硫醚连接，次级偶联至特别的包含自由巯基的分子；用于制备酶的免疫交联物以及半抗原载体分子结合体，包含了9个原子连接；一种扩展的脂肪质的间隔片，与芳香族间隔片相比，使顺丁烯二酰亚胺稳定地优先偶联
