中文名称:
2,2-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐
中文同义词:
ABTS [=2,2 -联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐][用于生化研究];2,2-联氮基双(3-乙基苯并噻唑啉-6-磺酸)二铵盐;2,2-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS);乙基苯并噻唑-6-磺酸铵盐;2,2-二氮-双(3-乙基苯并噻唑-6-磺酸)铵盐;2,2-二氮-双(3-乙基苯并噻唑-6-磺酸)铵盐/ABTS;2 2-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(+4℃);2,2-联氨-双(3-乙基苯并噻吡咯啉-6-磺酸)二胺盐
英文名称:
Diammonium 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
英文同义词:
ABTS DIAMMONIUM SALT;ABTS-(NH4)2;ABTS(R), DIAMMONIUM SALT;ABTS SUBSTRATE;ABTS(TM) CHROMOPHORE, DIAMMONIUM SALT;ABTS;2,2-AZINOBIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID AMMONIUM SALT);2,2-AZINO-BIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID) DIAMMONIUM SALT
相关类别:
中药对照品;化学试剂;生化试剂-色素类;生化试剂;有机化合物;色素类;其他生化试剂;无机化合物和盐;基因组学和分子诊断;稳定同位素;酶与辅酶;诊断试剂系列;自主产品;高纯生化试剂;优势产品;医用原料;医药原料;生物试剂;Biochemistry;Enzyme;Substrates;buffer;生物化工;化学试剂;生物化学;化学原料
溶解度
H2O: 50 mg/mL, very slightly hazy, green
PH值
pH(50g/l, 25℃) : 5.0~6.0
水溶解性
Dissolve in water at 50mg/ml. Solution may be slightly hazy.
CAS 数据库
30931-67-0(CAS DataBase Reference)
生物活性
ABTS diammonium salt 是一种辣根过氧化物酶 (HRP) 的底物。
体外研究
A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H 2 O 2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H 2 O 2 . Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 µM.
用途
一种适于ELISA步骤的过氧化物酶基质,这一基质产生可溶的绿色终产物,并可以利用分光光度计在405nm处观测到;游离氯的光谱试剂,酶免显色底物过氧化物酶的底物
用途
游离氯的光谱试剂,酶免显色底物,过氧化物酶的底物,用于ELISA 步骤,该底物产生一种可溶性的显绿色的终端产品可在405 nm处光谱读取。这种反应可用1%SDS终止。
可燃性危险特性
可燃; 燃烧产生有毒氮氧化物和硫氧化物烟雾