英文同义词:
Fukinolide;(-)-Bakkenolide B;(1R,3R,7aβ)-1β-Acetoxy-3aβ,4β-dimethyl-4-methylene-7α-[[(2Z)-2-methyl-2-butenoyl]oxy]spiro[oxolane-3,2-hydrindane]-2-one;(Z)-2-Methyl-2-butenoic acid [(3R,3aα)-3α-acetoxy-4,5,1,3,3a,4,5,6,7,7a-decahydro-7α,7aα-dimethyl-4-methylene-2-oxospiro[furan-3(2H),2-[2H]inden]-4β-yl] ester;Bakkenolide B
生物活性
Bakkenolide B 是从 petasites japonicas 叶片中分离出来的，具有抗过敏和抗炎作用。 Bakkenolide B 可用于研究哮喘。
体外研究
Bakkenolide B (0-100 μg/ml; pretreatment 30 mins) inhibits antigen-induced β-hexosamidase release from RBL2H3 mast cells in a concentration-dependent manner.Bakkenolide B (0-10 μg/ml; 1 h later stimulated with LPS) strongly inhibits the gene inductions of inducible nitric oxide synthase and cyclooxygenase-2 in a concentration-dependent manner in mouse peritoneal macrophages.
Western Blot Analysis
Cell Line: Mouse peritoneal macrophages
Concentration: 0 μg/ml; 1 μg/ml; 3 μg/ml; 5 μg/ml; 10 μg/ml
Incubation Time: 1 h later stimulated with LPS
Result: Decreased iNOS and cox-2 protein expression.
Cell Line:
Mouse peritoneal macrophages
Concentration:
0 μg/ml; 1 μg/ml; 3 μg/ml; 5 μg/ml; 10 μg/ml
Incubation Time:
1 h later stimulated with LPS
Result:
Decreased iNOS and cox-2 protein expression.
体内研究
Bakkenolide B (intraperitoneal injection; 1 mg/ml; 1 h before ovalbumin nebulization on days 28, 29, and 30) strongly inhibits the accumulation of eosinophils, macrophages, and lymphocytes to bronchoalveolar lavage fluid (BALF) in ovalbumin-induced asthma mice.
Animal Model: Ovalbumin-induced asthma mice
Dosage: 1 mg/ml
Administration: Intraperitoneal injection; 1 h before ovalbumin nebulization on days 28, 29, and 30
Result: Exhibited anti-allergic effects in mice.
Animal Model:
Ovalbumin-induced asthma mice
Administration:
Intraperitoneal injection; 1 h before ovalbumin nebulization on days 28, 29, and 30
Result:
Exhibited anti-allergic effects in mice.
