2-硝基苯-beta-D-半乳糖苷

中文名称:

2-硝基苯-beta-D-半乳糖苷

中文同义词:

2-硝基苯基-~-D-半乳糖苷, 98+%;邻硝基苯-Α-D-吡喃葡萄糖苷/2-硝基苯-Α-D-吡喃葡萄糖苷/ONPG;2-硝基苯基-Β-D-吡喃半乳糖苷(动物源);邻硝基苯-BETA-D-半乳糖苷;2-硝基苯基-Β-D-吡喃半乳糖苷(植物源);2-硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Β-D-半乳糖苷(ONPG)

英文名称:

2-Nitrophenyl-beta-D-galactopyranoside

英文同义词:

2-Nitrophenyl hexopyranoside;2-Nitrophenyl-beta-delta-galactopyranoside;2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol;o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem;beta-D-Galactopyranoside, 2-nitrophenyl;beta-d-galactopyranoside,2-nitrophenyl;Galactopyranoside, o-nitrophenyl, beta-D-;o-Nitrophenyl beta-galactoside

CAS号:

369-07-3

分子式:

C12H15NO8

分子量:

301.25

EINECS号:

206-716-1

相关类别:

化学试剂;分子生物试剂-多肽与蛋白;碳水化合物;专业试剂;酶类;中间体;糖类（碳水化合物）;糖类;检测标志试剂;酶底物;糖类化合物;氨基酸;核苷;糖苷系列;多肽与蛋白;酶活性检测;生化试剂-酶类;即用型溶液;Substrates;Biochemistry;Galactose;Glycosides;Sugars;Enzyme substrates;Carbohydrates Derivatives;生化试剂;substrate;酶及活性检测;试剂;优势产品;其他原料;精细化工原料;化学原料

Mol文件:

369-07-3.mol

熔点

186-193 °C (dec.)

比旋光度

-70 º (c=1, H2O)

沸点

442.39°C (rough estimate)

密度

1.3709 (rough estimate)

折射率

-68.8 ° (C=1, H2O)

储存条件

2-8°C

酸度系数(pKa)

12.69±0.70(Predicted)

形态

Powder

颜色

White to very slightly yellow

旋光性 (optical activity)

[α]20/D 70±1°, c = 1% in H2O

水溶解性

Soluble in water.

BRN

92207

InChIKey

KUWPCJHYPSUOFW-RMPHRYRLSA-N

CAS 数据库

369-07-3(CAS DataBase Reference)

NIST化学物质信息

Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3)

生物活性

ONPG 是一种比色和分光光度计底物，用于检测 β-半乳糖苷酶活性。

体外研究

The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (K m ), turnover numbers (k cat ), and catalytic efficiencies (k cat /K m ) of Gal308 for ONPG and lactose are determined. The k cat and K m values are 464.7±7.8 s -1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s -1 and 7.1±0.8 mM for lactose, respectively. The k cat /K m value of the enzyme for ONPG (172.1 s -1 mM -1 ) is 4.6-fold higher than that for lactose (37.2 s -1 mM -1 ), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose.

用途

用于测定β-半乳糖苷酶活性

用途

生化研究

用途

用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。

危险品标志

危险类别码

36/37/38

安全说明

22-24/25-36-26

WGK Germany