黄柏苷
中文名称:
黄柏苷
中文同义词:
二氢黄柏苷;黄柏甙;(二氢)黄柏甙;黄柏苷
英文名称:
phellamurin
英文同义词:
phellamurin;(2R)-7-(β-D-Glucopyranosyloxy)-2,3-dihydro-3β,5-dihydroxy-2α-(4-hydroxyphenyl)-8-(3-methyl-2-butenyl)-4H-1-benzopyran-4-one;(dihydro) Phellodendron;4H-1-Benzopyran-4-one,7-(b-D-glucopyranosyloxy)-2,3-dihydro-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-,(2R,3R)-;4H-1-Benzopyran-4-one, 7-(β-D-glucopyranosyloxy)-2,3-dihydro-3,5-dihydroxy-2-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-, (2R,3R)-
CAS号:
52589-11-4
分子式:
C26H30O11
分子量:
518.52
EINECS号:
相关类别:
标准品;植物提取物;标准品 -对照药材;黄酮
Mol文件:
52589-11-4.mol
沸点
861.5±65.0 °C(Predicted)
密度
1.504±0.06 g/cm3(Predicted)
酸度系数(pKa)
7.15±0.60(Predicted)
生物活性
Phellamurin 是一种来自 Phellodendron amurense 叶的植物黄酮糖苷，可抑制肠道 P-糖蛋白 (P-glycoprotein)。Phellamurin 还抑制凤蝶的产卵。Phellamurin 诱导细胞凋亡 (apoptosis)，并具有抗肿瘤活性。
体外研究
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment leads to a repression of cell viability in U2OS and Saos-2 cells in a dose-dependent manner.
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment concentration-dependently promots the apoptosis of U2OS and Saos-2 cells.
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment declines the ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR in U2OS and Saos-2 cells.
Cell Viability Assay
Cell Line: U2OS and Saos-2 cells
Concentration: 0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL
Incubation Time: 48 hours
Result: Suppressed the viability of U2OS and Saos-2 cells in a concentration-dependent manner.
Apoptosis Analysis
Cell Line: U2OS and Saos-2 cells
Concentration: 0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL
Incubation Time: 48 hours
Result: Induced apoptosis of U2OS and Saos-2 cells in a concentration-dependent manner.
Western Blot Analysis
Cell Line: U2OS and Saos-2 cells
Concentration: 0 μg/mL and 10 μg/mL
Incubation Time: 48 hours
Result: Repressed the PI3K/AKT/mTOR pathway in U2OS and Saos-2 cells.
Cell Line:
U2OS and Saos-2 cells
Concentration:
0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL
Incubation Time:
48 hours
Result:
Suppressed the viability of U2OS and Saos-2 cells in a concentration-dependent manner.
Cell Line:
U2OS and Saos-2 cells
Concentration:
0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL
Incubation Time:
48 hours
Result:
Induced apoptosis of U2OS and Saos-2 cells in a concentration-dependent manner.
Cell Line:
U2OS and Saos-2 cells
Concentration:
0 μg/mL and 10 μg/mL
Incubation Time:
48 hours
Result:
Repressed the PI3K/AKT/mTOR pathway in U2OS and Saos-2 cells.
体内研究
Phellamurin (50 mg/kg/day; intraperitoneal injection; daily; for 21 days; female BALB/c nude mice) treatment represses osteosarcoma tumor growth in vivo. The ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR are decreased in xenograft in Phellamurin-treated mice.
Animal Model: Athymic female BALB/c nude mice (6 weeks old ) injected with U2OS cells
Dosage: 50 mg/kg/day
Administration: Intraperitoneal injection; daily; for 21 days
Result: Repressed osteosarcoma tumor growth in vivo.
Animal Model:
Athymic female BALB/c nude mice (6 weeks old ) injected with U2OS cells
Dosage:
50 mg/kg/day
Administration:
Intraperitoneal injection; daily; for 21 days
Result:
Repressed osteosarcoma tumor growth in vivo.
