黄羽扇豆魏特酮
中文名称:
黄羽扇豆魏特酮
中文同义词:
黄羽扇豆魏特酮
英文名称:
8-PRENYLGENISTEIN
英文同义词:
8-PRENYLGENISTEIN;5,7-Dihydroxy-3-(4-hydroxyphenyl)-8-(3-methyl-2-butenyl)-4H-1-benzopyran-4-one;3-(4-Hydroxyphenyl)-5,7-dihydroxy-8-(3-methyl-2-butenyl)-4H-1-benzopyran-4-one;4,5,7-Trihydroxy-8-isopentenylisoflavone;4,5,7-Trihydroxy-8-prenylisoflavone;8-Isopentenylgenistein;Lupiwighteone;4H-1-Benzopyran-4-one, 5,7-dihydroxy-3-(4-hydroxyphenyl)-8-(3-methyl-2-buten-1-yl)-
CAS号:
104691-86-3
分子式:
C20H18O5
分子量:
338.35
EINECS号:
相关类别:
黄酮;标准品;Miscellaneous Natural Products
Mol文件:
Mol File
熔点
133-135℃
生物活性
Lupiwighteone 是一种广泛存在于野生植物中的异黄酮，具有抗氧化、抗菌和抗癌作用。Lupiwighteone 通过抑制 PI3K/Akt/mTOR 通路，诱导人乳腺癌细胞 caspase 依赖性和非依赖性凋亡 (apoptosis)。
靶点
apoptosis
体外研究
Lupiwighteone (2-100 μM; 72 hours) shows cytotoxicity towards various cell lines, especially on DU-145 cells and SGC-7901 cells with IC 50 s of 23.7 μM and 21 μM, respectively.
Lupiwighteone (20-60 μM; 48 hours) induces (cell cycle arrest in DU-145 cells.
Lupiwighteone (20-60 μM; 48 hours) induces cells apoptosis.
Lupiwighteone (20-60 μM; 48 hours) decreases the cell cycle-related protein expressions in a dose-dependent manner in DU-145 cells.
Lupiwighteone (20-60 μM; 48 hours) induces a dose-dependent increase in ROS production.
Lupiwighteone up-regulates of cytochrome c and caspase-3, and subsequent cleavage of PARP-1 and down-regulates of the p-Akt/Akt ratio and VEGF expression, suggests the activation of mitochondria-based intrinsic apoptosis in DU-145 cells.
Cell Viability Assay
Cell Line: DU-145 cells, C4-2 cells, SGC-7901 cells, Hela cells, HUVEC, MCF-7 cells, CNE cells, Vero
Concentration: 2 μM, 5 μM, 25 μM, 50 μM, 100 μM
Incubation Time: 72 hours
Result: Had inhibition on the survival of many cancer cell lines and HUVEC.
Cell Cycle Analysis
Cell Line: DU-145 cells
Concentration: 20 μM, 40 μM, 60 μM
Incubation Time: 48 hours
Result: Induced cell cycle arrest.
Apoptosis Analysis
Cell Line: DU-145 cells
Concentration: 20 μM, 40 μM, 60 μM
Incubation Time: 48 hours
Result: Induced apoptosis.
Western Blot Analysis
Cell Line: DU-145 cells
Concentration: 20 μM, 40 μM, 60 μM
Incubation Time: 48 hours
Result: Decreased CDK1, 2, 4, 6, cyclinD1, and cyclinB1 protein expression in a dose-dependent manner.
Cell Line:
DU-145 cells, C4-2 cells, SGC-7901 cells, Hela cells, HUVEC, MCF-7 cells, CNE cells, Vero
Concentration:
2 μM, 5 μM, 25 μM, 50 μM, 100 μM
Incubation Time:
72 hours
Result:
Had inhibition on the survival of many cancer cell lines and HUVEC.
Cell Line:
DU-145 cells
Concentration:
20 μM, 40 μM, 60 μM
Incubation Time:
48 hours
Result:
Induced cell cycle arrest.
Cell Line:
DU-145 cells
Concentration:
20 μM, 40 μM, 60 μM
Incubation Time:
48 hours
Result:
Induced apoptosis.
Cell Line:
DU-145 cells
Concentration:
20 μM, 40 μM, 60 μM
Incubation Time:
48 hours
Result:
Decreased CDK1, 2, 4, 6, cyclinD1, and cyclinB1 protein expression in a dose-dependent manner.
