中文名称:

荧光素

中文同义词:

荧光橙红;荧光素, 指示剂;黄 7 [CI 45350:1];荧光素,水溶;荧光酯;萤光橙黄;萤光红;萤光黄

英文名称:

Fluorescein

英文同义词:

FLUORESCEIN, FREE ACIDFLUORESCEIN, FREE ACIDFLUORESCEIN, FREE ACID;Diresorcinolphthalein;Tetraoxyphthalophenone anhydride;SOLVENT YELLOW 94;S NO 880;RESORCINOL PHTHALEIN;FLUORESCEIN, ALCOHOL SOLUBLE;FLUORESCEIN

CAS号:

2321-07-5

分子式:

C20H12O5

分子量:

332.31

EINECS号:

219-031-8

相关类别:

滴定;分析试剂;标准品;色素类;生化试剂;生物染色剂;小分子抑制剂;Aromatics;Fluorescent Labels Indicators;Heterocycles;FUNDUSCEIN;基因组学和分子诊断;Fluorescent;Xanthene;化合物;化学试剂;生化试剂-色素类;化工原料;大化工;化工材料;有机试剂;E-FFluorescent Probes, Labels, Particles and Stains;Fluorescein and Derivatives;Fluorescent Labels;Stains and Dyes;StainsDyes, A to;Intermediates Fine Chemicals;Pharmaceuticals

Mol文件:

2321-07-5.mol

熔点 

320 °C(lit.)

沸点 

429.44°C (rough estimate)

密度 

1.2739 (rough estimate)

折射率 

1.5000 (estimate)

储存条件 

room temp

溶解度 

Solubility Insoluble in water, ether, benzene, chloroform; soluble in ethanol, methanol, acetone, Iethyl acetate, N,N-dimethylformamide

色指数 

45350

形态

Powder

酸度系数(pKa)

2.2, 4.4, 6.7(at 25℃)

颜色

Red to orange

酸碱指示剂变色ph值范围

Pink uorescence (4.0) to green uorescence (6.0)

水溶解性 

insoluble

最大波长(λmax)

493.5nm, 496nm, 460nm, 515nm

Merck 

14,4159

BRN 

94324

稳定性

Stable. Combustible. Incompatible with strong oxidizing agents.

生物领域应用

Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity

主要应用

Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer

CAS 数据库

2321-07-5(CAS DataBase Reference)

EPA化学物质信息

Fluorescein (2321-07-5)

生物活性

Fluorescein (Resorcinol phthalein) 是一种合成的有机光敏染料化合物。Fluorescein 是荧光示踪剂，可以应用于医学和生物学以及肿瘤感染的组织。

体外研究

Fluorescein is nearly inactive as a photodynamic sensitizer. Fluorescein consequently is almost useless as a photodynamic sensitizer, but it is of great value in fluorescence immunoassays. The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent.

化学性质 

橙黄或浅橙红色结晶粉末。熔点314-316℃（分解）。溶于热乙醇、冰醋酸、碱溶液，呈绿色萤光，不溶于水、苯、氯仿和乙醚。

用途 

汞溴红的中间体。萤光光度分析试剂，测定氯、溴、碘、硫离子；氧化-还原指示试剂；生物染色剂。

用途 

荧光光度分析硫离子，试验溴，滴定氯、溴和碘。吸附指示剂，氧化还原指示剂。

用途 

吸附指示剂；氧化还原指示剂；荧光光度分析硫离子；滴定氯、溴和碘

生产方法 

用邻苯二甲酸酐与间苯二酚在硫酸参与下反应制得。

危险品标志 

Xi

危险类别码 

43-36-36/37/38

安全说明 

22-24/25-37/39-26-36/37/39-27

WGK Germany 

3

RTECS号

LM5075000

F 

10-21

TSCA 

Yes

海关编码 

32042000

毒害物质数据

2321-07-5(Hazardous Substances Data)