血根碱

中文名称:

血根碱

中文同义词:

血根碱硫酸盐;氯化血根碱(标准品);血根碱氯;血根碱, ≥98%(HPLC);氯化血根碱/盐酸血根碱;血根碱水合物;盐酸血根碱, 来源于博落回;盐酸血根碱(氯化血根碱)

英文名称:

SANGUINARINE CHLORIDE

英文同义词:

SANGUINARINE CHLORIDE;SANGUINARINE HCL;PSEUDOCHELERYTHRINE;PSEUDOCHELERYTHRINE CHLORIDE;13-METHYL-[1,3]BENZODIOXOLO[5,6-C]-1,3-DIOXOLO[4,5-I] PHENANTHRIDINIUM CHLORIDE;Sanguinarine chloride, froM Macleaya cordata;[1,3]Benzodioxolo[5,6-c]-1,3-dioxolo[4,5-i]phenanthridinium,13-methyl-, chloride (1:1);3)benzodioxolo(5,6-c)-1,3-dioxolo(4,5-i)phenanthridinium,13-methyl-(chlo

CAS号:

5578-73-4

分子式:

C20H14ClNO4

分子量:

367.78

EINECS号:

相关类别:

分析标准品;标准品;植物提取物;植物生化提取物;中药标准品;生物碱;植提;植提标准品;碱;小分子抑制剂;小分子抑制剂，天然产物;医药原料;其他生化试剂;分析对照品;分析试剂标准品;标准品-对照品;对照品-中药对照品;对照品;标准品 -中药标准品;分析试剂-标准品;中药对照品;Alkaloids;AlkaloidCancer Research;Anti-proliferative AgentsVoltage-gated Ion Channels;Ion Pump Inhibitors;Biochemicals Found in Plants;Chemopreventive Agents;Monovalent Ion Channels;Nutrition Research;Amines;Aromatics;Heterocycles;Intermediates Fine Chemicals;Pharmaceuticals;VIADENT;Inhibitors

Mol文件:

5578-73-4.mol

熔点

281-285°C

储存条件

Inert atmosphere,2-8°C

溶解度

methanol: 20 mg/mL, clear, orange

形态

Orange solid.

生物活性

Sanguinarine (Sanguinarin) chloride 是一种来源于 Sanguinaria Canadensis 的生物碱，可通过激活活性氧 (ROS) 的产生来刺激细胞凋亡。Sanguinarine 诱导的细胞凋亡与 JNK 和 NF-κB 的活化有关。

靶点

Target Value PP2C (Cell-free) 0.68 μM(Ki)

Target

Value

PP2C (Cell-free)

0.68 μM(Ki)

体外研究

Sanguinarine (SANG)-induced apoptosis is associated with the activation of JNK and NF-κB signal pathways.To determine the effects of Sanguinarine on cell viability, 22B-cFluc cells are stimulated with different concentrations of Sanguinarine for 24 h, and then a CKK-8 assay is performed. The treatment with Sanguinarine decreases the proliferation of 22B cells in a dose-dependent manner. Meanwhile, the cytosolic extracts of 22B-cFluc cells treated with different dose of Sanguinarine are measured to detect cellular caspase-3 activity using Ac-DEVD-pNA, which is a validated caspase-3 substrate. The absorbance at 450 nm increases in a dose-dependent manner, indicating increased caspase-3 activity stimulated by Sanguinarine.

体内研究

To evaluate the apoptosis induced by Sanguinarine (SANG) in vivo, 22B-cFluc cells are inoculated subcutaneously into one flank of nude mice and xenograft models are allowed to establish. Mice are treated intravenously with 10 mg/kg of Sanguinarine. At 24, 48 and 72 h after treatment, bioluminescent imaging is performed after i.p. injection of mice with 150 mg/kg of D-luciferin substrate. Sanguinarine treatment induces an obvious increase of luminescent signal as early as 48 h after initial treatment. A sustained bioluminescent imaging (BLI) intensity increased is observed throughout the course of experiment. At 72 h after treatment, the tumors are collected and subjected to TUNEL staining for evaluating apoptosis. Compared with the control tumors, the group treated with Sanguinarine exhibits significantly more cell apoptosis, indicated by the increased green signals from the sporadic apoptotic cells.

化学性质

红色针状结晶，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于博落回。

用途

一个具有抗菌，消炎，抗氧化性能的天然产物。有抑制肿瘤细胞株扩增和促凋亡作用。

危险品标志

危险类别码

安全说明

36-26

WGK Germany

RTECS号

VP5220000

海关编码