中文同义词:
PRMT5抑制剂(HLCL-61 HYDROCHLORIDE);HLCL-61 盐酸盐
英文名称:
HLCL-61 (hydrochloride)
英文同义词:
HLCL-61 (hydrochloride);HLCL-61.HCl;HLCL61;HLCL 61;CS-2798;HLCL61;HLCL 61;HLCL 61 HYDROCHLORIDE
储存条件
Inert atmosphere,Store in freezer, under -20°C
生物活性
HLCL-61 hydrochloride 是首创的小分子蛋白精氨酸甲基转移酶 5 (PRMT5) 抑制剂。
靶点
Protein arginine methyltransferase 5
体外研究
HLCL-61 hydrochloride (1-100 μM; 24-72 hours) reduces cells growth at dose-dependent manner with IC 50 s of 14.12, 16.74, 6.3, 8.72 μM for MV4-11 cells, THP-1 cells, FLT3-WT blast, and FLT3-ITD blast, respectively.
HLCL-61 hydrochloride shows effective inhibition of symmetric arginine dimethylation (me2) of histones H3 and H4 in AML samples, starting at 12 h post-treatment and persisting after 48 h.
Cell Viability Assay
Cell Line: MV4-11 cells, THP-1 cells, FLT3-WT blast (primary blasts from patients), FLT3-ITD blast (primary blasts from patients)
Concentration: 1, 5, 10, 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Dose-dependent reduction in cell viability with IC 50 s of 14.12, 16.74, 6.3, 8.72 μM for MV4-11 cells, THP-1 cells, FLT3-WT blast, and FLT3-ITD blast, respectively.
Cell Line:
MV4-11 cells, THP-1 cells, FLT3-WT blast (primary blasts from patients), FLT3-ITD blast (primary blasts from patients)
Concentration:
1, 5, 10, 25, 50, 100 μM
Incubation Time:
24, 48, 72 hours
Result:
Dose-dependent reduction in cell viability with IC 50 s of 14.12, 16.74, 6.3, 8.72 μM for MV4-11 cells, THP-1 cells, FLT3-WT blast, and FLT3-ITD blast, respectively.